Supporting Material: Active generation and propagation of Ca signals within tunneling membrane nanotubes
نویسندگان
چکیده
CELL CULTURE: Human neuroblastoma SH-SY5Y cells were cultured in a mixture of Ham’s F12 medium and Eagle’s minimal essential medium (1:1 mixture), supplemented with 10% (v/v) fetal calf serum and 1% nonessential amino acids. Cells were incubated at 37 °C in a humidified incubator gassed with 95% air and 5% CO2, passaged every 7 days and used for up to 20 passages. Cells were harvested in phosphate-buffered saline (PBS) without Ca 2+ or Mg 2+
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Active generation and propagation of Ca2+ signals within tunneling membrane nanotubes.
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